{"id":79685,"date":"2024-04-19T09:40:30","date_gmt":"2024-04-19T07:40:30","guid":{"rendered":"https:\/\/aktuelles.uni-frankfurt.de\/?p=79685"},"modified":"2024-04-19T10:05:26","modified_gmt":"2024-04-19T08:05:26","slug":"double-barrier-to-antibiotics","status":"publish","type":"post","link":"https:\/\/aktuelles.uni-frankfurt.de\/en\/english\/double-barrier-to-antibiotics\/","title":{"rendered":"Double barrier to antibiotics"},"content":{"rendered":"

Two scientists at Goethe University Frankfurt are seeking weaknesses in sophisticated bacterial defense systems. Their goal: Find new approaches to combat bacterial infections \u2013 something they will also be focusing on as part of the SCALE cluster initiative.<\/strong><\/p>\n\n\n\n

Transport proteins are membrane proteins that perform the special task of ensuring that substances can pass between the cell\u2019s interior and the extracellular space. One large family of these transporters is known as ABC transporters, whereby ABC stands for ATP-binding cassettes. Their characteristic feature is that they split energy-carrying adenosine triphosphate (ATP) molecules to release the energy they need to actively transport substances through the cell membrane. This characteristic classifies them as part of the family of primary active transporters.<\/p>\n\n\n\n

Clemens Glaubitz from Goethe University\u2019s Institute of Biophysical Chemistry is researching ABC proteins that regulate transport in bacteria. He specializes in Gram-negative bacteria, which include Escherichia coli<\/em>, Klebsiella<\/em> and Acinetobacter baumannii,<\/em> and which possess not only one membrane surrounding and protecting the cell contents \u2013 the cell membrane \u2013 but also have a second outer membrane separated from the inner one by the periplasm. The two membranes are constructed differently: whereas the inner membrane comprises a symmetrical lipid bilayer, the outer membrane is asymmetrical and contains complex compounds consisting of fatty acids and lipopolysaccharides (LPS), i.e. polysaccharides. \u201cThese are the most important components of the outer membrane. They help maintain the bacteria\u2019s cellular architecture,\u201d Glaubitz explains.<\/p>\n\n\n\n

<\/div>\n\n\n\n

SCALE \u2013 Subcellular Architecture of Life
<\/strong>Cells consist of billions of molecules, organized as individual molecules, large molecular complexes or whole organelles. While the functions of many individual molecules are known, in many cases it remains unclear how the architecture inside a cell arises and functions, and how its various parts interact. To gain a better understanding of how cells really function and how their various \u201cmachines\u201d cooperate, the scientists at SCALE want to discover the principles of cellular self-organization and create a simulation of the cell in high spatial and temporal resolution. On February\u00a02, 2024, the German Research Foundation (DFG) announced that, based on its draft concept, SCALE may submit a full proposal in August 2024 for funding as a Cluster of Excellence from 2026. https:\/\/scale-frankfurt.org<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

However, for LPS to fulfill their stabilizing function, they first have to actually arrive at the outer membrane. This vital task falls to the ABC transporters, which bring the polysaccharides from the cell\u2019s interior, where they are produced, through the cell membrane and the periplasm, to their destination on the outer membrane. Glaubitz\u2019 lab is currently investigating the ABC transporter MsbA and the transport system linked to this protein, which creates a kind of bridge from the inner membrane to the outer one \u2013 which the polysaccharides just have to flow across in the outer membrane\u2019s direction. Glaubitz intends to use Escherichia coli<\/em>, a bacterium that lives in the gut, to find out how the whole process is controlled.<\/p>\n\n\n\n

<\/div>\n\n\n\n

New targets for antibiotics<\/h4>\n\n\n\n

The experimental method applied by his team is known as solid-state NMR spectroscopy. Whereas nuclear magnetic resonance spectroscopy usually requires the samples under investigation to be soluble, when the technique is expanded to non-soluble samples, we speak of solid-state NMR spectroscopy. The researchers in Glaubitz\u2019 lab discovered that MsbA \u2013 and its related ABC transporters in Gram-negative bacteria \u2013 seem to adapt the transportation of polysaccharides to changes in the environment. This means that the LPS transport system can become more efficient as and when necessary. Glaubitz plans to use MsbA as a model protein for all other such systems in fellow Gram-negative bacteria, including Acetinobacter baumannii<\/em>. Asked where this basic research could lead to, he says that in the future, LPS-transporting proteins like MsbA could become new targets in the treatment of bacterial infections using antibiotics. \u201cMaybe one day we\u2019ll be able to inhibit the proteins involved in transporting LPS.\u201d Unable to reach the outer cell membrane, they would no longer be able to stabilize the bacterial architecture.<\/p>\n\n\n\n

<\/div>\n\n\n\n
\"\"
The antibiotic efflux pump<\/strong>
The antibiotic efflux pump Klaas Martinus Pos is researching also appears to determine the correct construction of the bacterial cell envelope. The architecture of the cell envelope must be intact for bacteria to be resistant to antibiotics. This is why, as part of his work at SCALE, Pos is working together with other working groups to examine a number of bacterial cell-envelope architectures. \u201cThis is an exciting area about which we don\u2019t know very much yet. Maybe we\u2019ll uncover new ways of combating antibiotic-resistant bacteria in the future.\u201d Also as part of SCALE, Clemens Glaubitz is investigating the transport proteins that help maintain the double membrane in Gram-negative bacteria. He is focusing on bacterial lipid vesicles discharged from the outer membrane, which appear to be directly connected with its resistance and stress response mechanisms. \u201cIn addition, we\u2019re making our experience in solid-state NMR available to SCALE \u2013 a method that has proven its worth when it comes to analyzing proteins and lipids in membranes.\u201d<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

There is another reason why preventing the transport of polysaccharides outside the cell would be important for combating pathogens: LPS also form an initial external barrier against antibiotics. They do so because the polysaccharides are hydrophilic, i.e. attracted to water, while antibiotics are hydrophobic and therefore attracted to fats. This is why the latter generally cannot permeate the outer membrane. If they do manage to cross it, they are immediately met by a second line of defense, which Klaas Martinus Pos is investigating at Goethe University\u2019s Institute of Biochemistry. He focuses on the RND family of transporters, whereby RND stands for resistance-nodulation division. Embedded in the bacteria\u2019s inner cell membrane, these transporters belong to the family of secondary active transporters, as they use the electrochemical gradients of ions to supply the energy needed to drive active transport.<\/p>\n\n\n\n

Pumps of this type act like sentries that prevent antibiotics from diffusing through the inner cell membrane into the cytoplasm. Any intruding molecules are tackled before they get that far, and are exported out of the cell and back into the environment. During this process, the ABC transporters use what is known as the antiport mechanism, Pos explains: \u201cThey transport the ions in one direction, the cell\u2019s interior, and the toxic substance in the opposite direction, its exterior.\u201d Po researches Escherichia coli<\/em> and Klebsiella pneumoniae<\/em> bacteria, among others, both of which rely on a tripartite efflux pump called AcrAB-TolC, whose operation his lab has discovered: While AcrB is the actual RND transporter, AcrA is an adaptor protein, located in the periplasm, which connects the RND transporter to the third pump component, the pore TolC in the outer membrane. All three together form a long, flexible tunnel system, through which antibiotics are exported out of the cell. \u201cThis tripartite efflux pump is hugely efficient. It recognizes almost all known antibiotics and pumps them out.\u201d Scientists also speak of a multidrug efflux pump, which is the sole factor responsible for certain bacteria\u2019s resistance to a large number of antibiotics (a phenomenon known as multi-resistance).<\/p>\n\n\n\n

Pos and his team have also discovered how the efflux pump obtains the necessary energy. It binds protons from the periplasm and releases them into the cytoplasm. \u201cThe resulting electrostatic changes in the pump\u2019s membrane component ensure that the antibiotic molecules in the tunnel system move in one direction only, to the outside.\u201d The flexible tunnel mechanism resembles a peristaltic pump like the one found in our esophagus, which enables us to eat solid food even while standing on our heads: The food we swallow arrives in the stomach despite the opposing force of gravity. The bacterial efflux pump uses the same mechanism to prevent the antibiotics from sliding back into the cell during the expulsion process.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Snapshots of the efflux pump<\/h4>\n\n\n\n

Finding out where and how antibiotics are intercepted and expelled requires high-resolution structures, which \u2013 in the ideal case \u2013 make individual atoms visible. Pos\u2019 lab deploys specially grown crystals to create electron density maps, which are then used to build 3-D structures of the tripartite pumps \u2013 a process that can also be done using single-particle cryo-electron microscopy. \u201cBoth methods supply snapshots, individual images of the efflux pump at work, which we can view one after the other. It\u2019s like a flipbook \u2013 we can actually observe the antibiotic being pumped through the tunnel.\u201d<\/p>\n\n\n\n

Working together with Prof. Achilleas Frangakis\u2019 working group and the Institut Pasteur in Lille, Pos\u2019 team used snapshots to develop new kinds of inhibitors, i.e. substances that specifically hinder the multi-drug efflux pump. \u201cThis could enable us to once again effectively fight bacterial infections using existing antibiotics to which bacteria have already exhibited resistance.\u201d The effects of the inhibitors were recently demonstrated in a pulmonary model in mice infected with Klebsiella pneumoniae<\/em>. In December 2023, Clara B\u00f6rnsen and Reinke M\u00fcller, two researchers from Pos\u2019 and Frangakis\u2019 working groups, were awarded Goethe University\u2019s Unibator Innovation prize for their work on these inhibitors as part of the \u201cAntibiotics reloaded\u201d project.  <\/p>\n\n\n\n

Basic research into bacterial resistance mechanisms is urgently needed, Pos says. \u201cAt present, 1.3 million people around the world are killed by multi-resistant pathogens every year. If we don\u2019t act now, by 2050 we can expect ten million deaths a year.\u201d That would make multi-resistant pathogens even deadlier than cancer is today.<\/p>\n\n\n\n

Author: Andreas Lorenz-Meyer<\/em><\/p>","protected":false},"excerpt":{"rendered":"

Two scientists at Goethe University Frankfurt are seeking weaknesses in sophisticated bacterial defense systems. Their goal: Find new approaches to combat bacterial infections \u2013 something they will also be focusing […]<\/p>\n","protected":false},"author":8,"featured_media":79609,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_eb_attr":"","_price":"","_stock":"","_tribe_ticket_header":"","_tribe_default_ticket_provider":"","_ticket_start_date":"","_ticket_end_date":"","_tribe_ticket_show_description":"","_tribe_ticket_show_not_going":false,"_tribe_ticket_use_global_stock":"","_tribe_ticket_global_stock_level":"","_global_stock_mode":"","_global_stock_cap":"","_tribe_rsvp_for_event":"","_tribe_ticket_going_count":"","_tribe_ticket_not_going_count":"","_tribe_tickets_list":"[]","_tribe_ticket_has_attendee_info_fields":false,"footnotes":""},"categories":[126,254],"tags":[303,332],"post_folder":[],"class_list":["post-79685","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-english","category-research","tag-scale","tag-unireport-2-24"],"yoast_head":"\nDouble barrier to antibiotics | Aktuelles aus der Goethe-Universit\u00e4t Frankfurt<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/aktuelles.uni-frankfurt.de\/en\/english\/double-barrier-to-antibiotics\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Double barrier to antibiotics | Aktuelles aus der Goethe-Universit\u00e4t Frankfurt\" \/>\n<meta property=\"og:description\" content=\"Two scientists at Goethe University Frankfurt are seeking weaknesses in sophisticated bacterial defense systems. Their goal: Find new approaches to combat bacterial infections \u2013 something they will also be focusing […]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/aktuelles.uni-frankfurt.de\/en\/english\/double-barrier-to-antibiotics\/\" \/>\n<meta property=\"og:site_name\" content=\"Aktuelles aus der Goethe-Universit\u00e4t Frankfurt\" \/>\n<meta property=\"article:published_time\" content=\"2024-04-19T07:40:30+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-04-19T08:05:26+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/aktuelles.uni-frankfurt.de\/wp-content\/uploads\/2024\/04\/01_SCALE_2_650x450px.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"650\" \/>\n\t<meta property=\"og:image:height\" content=\"450\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"-\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"-\" \/>\n\t<meta name=\"twitter:label2\" content=\"Estimated reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"8 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/\"},\"author\":{\"name\":\"-\",\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/#\\\/schema\\\/person\\\/8e55ea338fb65d1ce87a91565d1f1739\"},\"headline\":\"Double barrier to antibiotics\",\"datePublished\":\"2024-04-19T07:40:30+00:00\",\"dateModified\":\"2024-04-19T08:05:26+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/\"},\"wordCount\":1551,\"publisher\":{\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/wp-content\\\/uploads\\\/2024\\\/04\\\/01_SCALE_2_650x450px.jpg\",\"keywords\":[\"SCALE\",\"UniReport 2.24\"],\"articleSection\":[\"English\",\"Research\"],\"inLanguage\":\"en-GB\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/\",\"url\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/\",\"name\":\"Double barrier to antibiotics | Aktuelles aus der Goethe-Universit\u00e4t Frankfurt\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/wp-content\\\/uploads\\\/2024\\\/04\\\/01_SCALE_2_650x450px.jpg\",\"datePublished\":\"2024-04-19T07:40:30+00:00\",\"dateModified\":\"2024-04-19T08:05:26+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/#primaryimage\",\"url\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/wp-content\\\/uploads\\\/2024\\\/04\\\/01_SCALE_2_650x450px.jpg\",\"contentUrl\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/wp-content\\\/uploads\\\/2024\\\/04\\\/01_SCALE_2_650x450px.jpg\",\"width\":650,\"height\":450,\"caption\":\"Die Antibiotika-Efflux-Pumpe Die Antibiotika-Efflux-Pumpe, die Klaas Martinus Pos untersucht, bestimmt vermutlich auch den korrekten Zellh\u00fcllenaufbau von Bakterien. Die intakte Architektur der Zellh\u00fclle ist Voraussetzung daf\u00fcr, dass Bakterien gegen Antibiotika resistent sein k\u00f6nnen. Innerhalb von SCALE untersucht Pos daher zusammen mit anderen Arbeitsgruppen unterschiedliche bakterielle Zellh\u00fcllenarchitekturen. \u201eEine spannende Sache, da wir noch nicht viel dar\u00fcber wissen. Vielleicht \u00f6ffnen sich so auch neue Wege, um Antibiotika-resistente Bakterien in Zukunft zu bek\u00e4mpfen.\u201c Clemens Glaubitz untersucht im Rahmen von SCALE die Transportproteine, die bei Gram-negativen Bakterien helfen, die Doppelmembran aufrechtzuerhalten. Im Blick hat er dabei besonders Lipidvesikel, die von den Bakterien aus der \u00e4u\u00dferen Membran abgesondert werden und direkt mit deren Resistenz-und Stressantwortmechanismen zusammenzuh\u00e4ngen scheinen. \u201eZudem stellen wir f\u00fcr SCALE unsere Erfahrung im Bereich Festk\u00f6rper-NMR zu Verf\u00fcgung, da sich diese Methode f\u00fcr die Analyse von Proteinen und Lipiden in Membranen sehr gut eignet.\u201c\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/english\\\/double-barrier-to-antibiotics\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Startseite\",\"item\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Double barrier to antibiotics\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/#website\",\"url\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/\",\"name\":\"Aktuelles aus der Goethe-Universit\u00e4t Frankfurt\",\"description\":\"Aktuelles aus der Goethe-Universit\u00e4t | Neues aus Forschung, Lehre, Studium\",\"publisher\":{\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-GB\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/#organization\",\"name\":\"Goethe-Universit\u00e4t\",\"url\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/800px-Goethe-Logo.png\",\"contentUrl\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/wp-content\\\/uploads\\\/2022\\\/03\\\/800px-Goethe-Logo.png\",\"width\":800,\"height\":436,\"caption\":\"Goethe-Universit\u00e4t\"},\"image\":{\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/#\\\/schema\\\/logo\\\/image\\\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/#\\\/schema\\\/person\\\/8e55ea338fb65d1ce87a91565d1f1739\",\"name\":\"-\",\"description\":\"Dieser Beitrag wurde von der Online-Redaktion ver\u00f6ffentlicht. Wenn der Beitrag von einem Gastautoren verfasst wurde, findet sich dieser Hinweis am Ende des jeweiligen Artikels.\",\"sameAs\":[\"http:\\\/\\\/aktuelles.uni-frankfurt.de\\\/autoren\"],\"url\":\"https:\\\/\\\/aktuelles.uni-frankfurt.de\\\/en\\\/author\\\/redaktion\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Double barrier to antibiotics | Aktuelles aus der Goethe-Universit\u00e4t Frankfurt","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/aktuelles.uni-frankfurt.de\/en\/english\/double-barrier-to-antibiotics\/","og_locale":"en_GB","og_type":"article","og_title":"Double barrier to antibiotics | Aktuelles aus der Goethe-Universit\u00e4t Frankfurt","og_description":"Two scientists at Goethe University Frankfurt are seeking weaknesses in sophisticated bacterial defense systems. Their goal: Find new approaches to combat bacterial infections \u2013 something they will also be focusing […]","og_url":"https:\/\/aktuelles.uni-frankfurt.de\/en\/english\/double-barrier-to-antibiotics\/","og_site_name":"Aktuelles aus der Goethe-Universit\u00e4t Frankfurt","article_published_time":"2024-04-19T07:40:30+00:00","article_modified_time":"2024-04-19T08:05:26+00:00","og_image":[{"width":650,"height":450,"url":"https:\/\/aktuelles.uni-frankfurt.de\/wp-content\/uploads\/2024\/04\/01_SCALE_2_650x450px.jpg","type":"image\/jpeg"}],"author":"-","twitter_card":"summary_large_image","twitter_misc":{"Written by":"-","Estimated reading time":"8 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/#article","isPartOf":{"@id":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/"},"author":{"name":"-","@id":"https:\/\/aktuelles.uni-frankfurt.de\/#\/schema\/person\/8e55ea338fb65d1ce87a91565d1f1739"},"headline":"Double barrier to antibiotics","datePublished":"2024-04-19T07:40:30+00:00","dateModified":"2024-04-19T08:05:26+00:00","mainEntityOfPage":{"@id":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/"},"wordCount":1551,"publisher":{"@id":"https:\/\/aktuelles.uni-frankfurt.de\/#organization"},"image":{"@id":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/#primaryimage"},"thumbnailUrl":"https:\/\/aktuelles.uni-frankfurt.de\/wp-content\/uploads\/2024\/04\/01_SCALE_2_650x450px.jpg","keywords":["SCALE","UniReport 2.24"],"articleSection":["English","Research"],"inLanguage":"en-GB"},{"@type":"WebPage","@id":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/","url":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/","name":"Double barrier to antibiotics | Aktuelles aus der Goethe-Universit\u00e4t Frankfurt","isPartOf":{"@id":"https:\/\/aktuelles.uni-frankfurt.de\/#website"},"primaryImageOfPage":{"@id":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/#primaryimage"},"image":{"@id":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/#primaryimage"},"thumbnailUrl":"https:\/\/aktuelles.uni-frankfurt.de\/wp-content\/uploads\/2024\/04\/01_SCALE_2_650x450px.jpg","datePublished":"2024-04-19T07:40:30+00:00","dateModified":"2024-04-19T08:05:26+00:00","breadcrumb":{"@id":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/"]}]},{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/#primaryimage","url":"https:\/\/aktuelles.uni-frankfurt.de\/wp-content\/uploads\/2024\/04\/01_SCALE_2_650x450px.jpg","contentUrl":"https:\/\/aktuelles.uni-frankfurt.de\/wp-content\/uploads\/2024\/04\/01_SCALE_2_650x450px.jpg","width":650,"height":450,"caption":"Die Antibiotika-Efflux-Pumpe Die Antibiotika-Efflux-Pumpe, die Klaas Martinus Pos untersucht, bestimmt vermutlich auch den korrekten Zellh\u00fcllenaufbau von Bakterien. Die intakte Architektur der Zellh\u00fclle ist Voraussetzung daf\u00fcr, dass Bakterien gegen Antibiotika resistent sein k\u00f6nnen. Innerhalb von SCALE untersucht Pos daher zusammen mit anderen Arbeitsgruppen unterschiedliche bakterielle Zellh\u00fcllenarchitekturen. \u201eEine spannende Sache, da wir noch nicht viel dar\u00fcber wissen. Vielleicht \u00f6ffnen sich so auch neue Wege, um Antibiotika-resistente Bakterien in Zukunft zu bek\u00e4mpfen.\u201c Clemens Glaubitz untersucht im Rahmen von SCALE die Transportproteine, die bei Gram-negativen Bakterien helfen, die Doppelmembran aufrechtzuerhalten. Im Blick hat er dabei besonders Lipidvesikel, die von den Bakterien aus der \u00e4u\u00dferen Membran abgesondert werden und direkt mit deren Resistenz-und Stressantwortmechanismen zusammenzuh\u00e4ngen scheinen. \u201eZudem stellen wir f\u00fcr SCALE unsere Erfahrung im Bereich Festk\u00f6rper-NMR zu Verf\u00fcgung, da sich diese Methode f\u00fcr die Analyse von Proteinen und Lipiden in Membranen sehr gut eignet.\u201c"},{"@type":"BreadcrumbList","@id":"https:\/\/aktuelles.uni-frankfurt.de\/english\/double-barrier-to-antibiotics\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Startseite","item":"https:\/\/aktuelles.uni-frankfurt.de\/"},{"@type":"ListItem","position":2,"name":"Double barrier to antibiotics"}]},{"@type":"WebSite","@id":"https:\/\/aktuelles.uni-frankfurt.de\/#website","url":"https:\/\/aktuelles.uni-frankfurt.de\/","name":"Aktuelles aus der Goethe-Universit\u00e4t Frankfurt","description":"Aktuelles aus der Goethe-Universit\u00e4t | Neues aus Forschung, Lehre, Studium","publisher":{"@id":"https:\/\/aktuelles.uni-frankfurt.de\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/aktuelles.uni-frankfurt.de\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-GB"},{"@type":"Organization","@id":"https:\/\/aktuelles.uni-frankfurt.de\/#organization","name":"Goethe University Frankfurt","url":"https:\/\/aktuelles.uni-frankfurt.de\/","logo":{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/aktuelles.uni-frankfurt.de\/#\/schema\/logo\/image\/","url":"https:\/\/aktuelles.uni-frankfurt.de\/wp-content\/uploads\/2022\/03\/800px-Goethe-Logo.png","contentUrl":"https:\/\/aktuelles.uni-frankfurt.de\/wp-content\/uploads\/2022\/03\/800px-Goethe-Logo.png","width":800,"height":436,"caption":"Goethe-Universit\u00e4t"},"image":{"@id":"https:\/\/aktuelles.uni-frankfurt.de\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/aktuelles.uni-frankfurt.de\/#\/schema\/person\/8e55ea338fb65d1ce87a91565d1f1739","name":"-","description":"Dieser Beitrag wurde von der Online-Redaktion ver\u00f6ffentlicht. Wenn der Beitrag von einem Gastautoren verfasst wurde, findet sich dieser Hinweis am Ende des jeweiligen Artikels.","sameAs":["http:\/\/aktuelles.uni-frankfurt.de\/autoren"],"url":"https:\/\/aktuelles.uni-frankfurt.de\/en\/author\/redaktion\/"}]}},"_links":{"self":[{"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/posts\/79685","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/comments?post=79685"}],"version-history":[{"count":0,"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/posts\/79685\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/media\/79609"}],"wp:attachment":[{"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/media?parent=79685"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/categories?post=79685"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/tags?post=79685"},{"taxonomy":"post_folder","embeddable":true,"href":"https:\/\/aktuelles.uni-frankfurt.de\/en\/wp-json\/wp\/v2\/post_folder?post=79685"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}